Journal: Stem cells (Dayton, Ohio)

Article Title: Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.

doi: 10.1634/stemcells.2007-0543

Figure Lengend Snippet: Figure 6. Binding and migration of human MSCs in vitro. (A): Binding of human MSCs to culture dishes coated with 10 g/ml of FN, CDH2, or NTRN1. The number of cells bound to FN-coated plates was significantly greater at all cell doses as compared with CDH2 or NTRN1-coated plates (p .0006). Preincubation of CDH2-coated plates with soluble CDH2 protein or (B) preincubation of MSCs with a neutralizing anti-NEO1 antibody (C) inhibited cell binding to CHD2 or NTRN1, respectively, in a dose-dependent fashion; , p 1 108. (D): Migration of human MSCs in response to SDF-1 and RGMA (25 g/ml) or VEGF165 and VEGF121 (10 g/ml) was significantly greater compared with medium alone; , p 5 109. Cell migration in response to RGMA was abolished by pretreatment of cells with a neutralizing anti-NEO1 antibody. (E): Migration of MSCs in response to VEGF165 was partially inhibited by preincubation of cells with anti-FLK1 and/or anti-FLT1 antibodies; , p .001; , p 1 109. (F): Migration of MSCs in response to VEGF121 was completely abolished by preincubating cells with anti-FLK1 and/or anti-FLT1 antibodies; , p 1 1011; , p 5 105. Abbreviations: CDH, cadherin; FN, fibronectin; NEO, neogenin; NTRN, netrin; OD, optical density; RGMA, repulsive guidance molecule A; SDF, stromal cell-derived factor; VEGF, vascular endothelial growth factor.

Article Snippet: Wells were precoated with 2–50 g/ml (200 l) recombinant mouse netrin 1, recombinant human cadherin 2, or bovine fibronectin (R&D Systems Inc., Minneapolis, http:// www.rndsystems.com) for 1 hour at 37°C.

Techniques: Binding Assay, Migration, In Vitro, Derivative Assay

Journal: STAR Protocols

Article Title: Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics

doi: 10.1016/j.xpro.2021.100954

Figure Lengend Snippet:

Article Snippet: Fibronectin bovine plasma , Merck , Cat#F4759-5MG.

Techniques: Recombinant, Modification, Transfection, Software, Inverted Microscopy, Microscopy, Cell Culture

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

doi: 10.1007/s00018-010-0349-z

Figure Lengend Snippet: Anchorage dependence: Colony-forming efficiency (a) as an index of malignancy was investigated in soft agar. Only tumor cells without anchorage dependence will grow. N2A cells were used as positive control and displayed a clearly detectable colony-forming potential. In contrast, neither primary nor immortalized HUVECs displayed colony-forming properties. ECM-dependent cell attachment: Experimental layout (b upper panel). Multiple substrate array (MSA) with 12 arrays of 64 microspots each. Each microspot represents one extracellular matrix (ECM) protein; 14 different ECM proteins in quadruplets. 1 PLL, 2 BSA, 3/4 fibronectin (FN), 5 vitronectin (VN), 6 heparan sulfate proteoglycan, 7/8 laminin, 9/10 collagen type I (C I), 11 collagen type II (C II), 12 collagen type III (C III), 13/14 collagen type IV (C IV), 15 collagen type V (C V), 16 collagen type VI (C VI). After cell seeding, incubation, fixation and DAPI staining the blue cell nuclei of attached cells were visualized (far right image, second row). Comparative MSA studies with both HUVECs (b lower panel). Strong adhesion is evident to most collagen types, weak adhesion to HSP and laminin. No adhesion to BSA. Primary and immortalized HUVECs displayed similar attachment characteristics. EHS Basement membrane of Engelbreth-Holm-Swarm murine sarcoma, hpc human placenta, hps human plasma, hFB human fibroblasts. Barrier characteristics of cell monolayers: Permeability measurements of 70 kDa FITC-dextran across monolayers of primary and hTERT-transduced HUVECs grown on different ECM components on transwell filters (c). There are essentially no differences between the two cell types. Cells grown on uncoated, collagen I, or fibronectin-coated filters showed higher permeability coefficients compared to cells cultivated on gelatin, indicating that both HUVEC cell types displayed best barrier integrity when cultivated on gelatin. *P < 0.05 with respect to control or PLL

Article Snippet: Bovine fibronectin (FN), human collagen I, TNFα, and interferon γ were purchased from Becton-Dickinson (BD, Heidelberg, Germany).

Techniques: Positive Control, Cell Attachment Assay, Incubation, Staining, Membrane, Clinical Proteomics, Permeability, Control

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

doi: 10.1007/s00018-010-0349-z

Figure Lengend Snippet: Permeability coefficients of both HUVEC types on different coated transwell filters using low (4 kDa) and high (70 kDa) molecular weight fluorescent dextran as tracer

Article Snippet: Bovine fibronectin (FN), human collagen I, TNFα, and interferon γ were purchased from Becton-Dickinson (BD, Heidelberg, Germany).

Techniques: Permeability, Molecular Weight, High Molecular Weight